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Rank difference between groups of TGFβ1 expression after IRI and normalised to a) 18S, b) GAPDH, c) PABPN1×HMBS, d) ACTB×PABPN1.

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posted on 2020-05-21, 17:41 authored by Sanjeeva Herath, Hongying Dai, Jonathan Erlich, Amy YM Au, Kylie Taylor, Lena Succar, Zoltán H. Endre

Data are means (one-way ANOVA) or medians (Kruskal Wallis) and 95% CI (n ≥ 6). * p < 0.05, ** p < 0.01, *** p < 0.001.

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Selection and validation of reference genes for normalisation of gene expression in ischaemic and toxicological studies in kidney disease
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candidate normalisation geneKIMACTBGAPDHglyceraldehyde 3- phosphate dehydrogenasekidney disease Normalisationtoxicological kidney modelsintra-class correlation coefficientgene expressionsample size calculation10 candidate reference genesPECAMpolymerase chain reactionPABPNgenes 18 STGFHIFGOIkidney cell typesReduced within-group variationreference genesreference gene stability

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