ppat.1008801.g010.tif (2.07 MB)
Download file

RSV infection induces the degradation of OsMYC2 by phosphorylation.

Download (2.07 MB)
figure
posted on 31.08.2020, 17:38 by Jinlong Hu, Jie Huang, Haosen Xu, Yongsheng Wang, Chen Li, Peizheng Wen, Xiaoman You, Xiao Zhang, Gen Pan, Qi Li, Hongliang Zhang, Jun He, Hongming Wu, Ling Jiang, Haiyang Wang, Yuqiang Liu, Jianmin Wan

(A) OsMYC2 protein levels in the RSV-infected and non-infected SYN rice plants. OsHSP indicates loading control. The number on the panel indicate the protein level of MBP-OsMYC2 relative to its initial value (0 h). The experiment was repeated four times. (B) Half-life plot for cell-free degradation of MBP-OsMYC2 in (A). (C) Phosphorylation levels of MBP-OsMYC2 incubated with the extracts from RSV-infected and non-infected rice plants using Phos-tag gels. The number under the panel indicate the ratio of pOsMYC2 versus OsMYC2. The experiment was repeated two times. (D) Determination of protein phosphorylation levels incubated with the extracts from rice plants collected at 0, 24 and 48 h post infection with RSV using Phos-tag gels. The number under the panel indicate the ratio of pOsMYC2 versus OsMYC2. The experiment was repeated three times. (E) Phosphorylation analysis of OsMYC2 in RSV-infected and non-infected plants using Phos-tag gels. The experiment was detected four independent RSV-infected and non-infected plants, respectively. (F) OsMYC2 protein levels in the RSV-infected and non-infected SYN rice plants. OsHSP indicates loading control. (G) The transcript levels of representative defense-related genes downstream of OsMYC2 in RSV-infected and non-infected SYN rice plants. AK058739 (subtilisin/chymotrypsin inhibitor), AK107891 (lipid transfer protein), AK109913 (similar to thaumatin-like protein) and AK060529 (beta-1, 3-glucanase). Data are shown as mean ± SEM (n > 3). *P < 0.05, **P < 0.01 by the Student’s t-test in B and G.

History