RNF43 truncating mutations have similar transcriptional profiles to BRAF missense mutations.

(A) LURE oncoprint of known driver BRAF missense mutations, which activate the MAPK/RTK pathways, used as “bait” in TCGA COAD. LURE finds RNF43 truncating events as a “catch” event [35]. (B) Kruskal Wallis test of LURE classifier scores across samples with different BRAF and RNF43 status. ns: 5.00e-02 < p < = 1.00e+00, *: 1.00e-02 < p < = 5.00e-02, **: 1.00e-03 < p < = 1.00e-02, ***: 1.00e-04 < p < = 1.00e-03, ****: p < = 1.00e-04) (C) Lollipop plots [20] showing the frequency RNF43-truncating mutations identified as catches (classifier score > 0.5) with LURE in the TCGA COAD cohort. The ZNRF-3 ectodomain is indicated in green and the ring finger domain is indicated in red (D) LURE classifier scores across the samples that have a single RNF43 truncating event and no co-occuring BRAF events (E) Model summarizing the predicted impacts of the RNF43 R117fs and RNF43 G659fs variants.