RNA expression patterns of ZmSTOMAGEN1/2 in wild-type maize and T3 transgenic seedling during early leaf development.

<p>(a) Wild-type maize seedlings 12 days after sowing. The yellow line represents the slice position in the dotted diagram. CO, coleoptile; LS, leaf sheath; wild-type (HiII-A × HiII-B). Cross section of leaf development from inside to outside around meristem apex in maize. The expression pattern of <i>ZmSTOMAGEN1</i> is gradually decreased with leaf development from inside (asterisk) to outside in wild type (b), and <i>ZmSTOMAGEN1</i> is more weakly expressed in transgenic line #1 (c) than in wild type plants. <i>ZmSTOMAGEN2</i> is strongly expressed in wild type (d) compared to in transgenic line #1 (e) Transcripts are undetectable in the sense control for <i>ZmSTOMAGEN1</i> and <i>ZmSTOMAGEN2</i> both in wild type (f, h) and transgenic line #1 (g, i). Asterisks indicate strong expression of <i>ZmSTOMAGEN1/2</i> was observed in the leaf around shoot apical meristem, but not in CO. Scale bars: 1.5 cm in a, 100 μm in b-i.</p>