RCAN1 effects mitochondrial membrane potential, not volume.
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posted on 2016-05-19, 10:54 authored by Heshan Peiris, Michael D. Duffield, Joao Fadista, Claire F. Jessup, Vinder Kashmir, Amanda J. Genders, Sean L. McGee, Alyce M. Martin, Madiha Saiedi, Nicholas Morton, Roderick Carter, Michael A. Cousin, Alexandros C. Kokotos, Nikolay Oskolkov, Petr Volkov, Tertius A. Hough, Elizabeth M. C. Fisher, Victor L. J. Tybulewicz, Jorge Busciglio, Pinar E. Coskun, Ann Becker, Pavel V. Belichenko, William C. Mobley, Michael T. Ryan, Jeng Yie Chan, D. Ross Laybutt, P. Toby Coates, Sijun Yang, Charlotte Ling, Leif Groop, Melanie A. Pritchard, Damien J. Keating(A) Representative western blots of wild type (WT) and RCAN1ox islet protein lysates probed against NDUFA9, SDHA, CORE1, Opa1 and TOM20. Electron microscopy analysis of (B) total mitochondrial area and (C) relative area in WT (n = 8 β-cells) and RCAN1ox (n = 6 β-cells). (D) TMRM staining in live cells further demonstrates a lack of difference in mitochondrial volume between WT (n = 43 cells) and RCAN1ox (n = 46 cells). (E) Mitochondrial membrane potential measured as mean cell TMRM fluorescence in low and high glucose in WT and RCAN1ox cells (n = 17–21 cell/group). Data represents the mean ±SEM, *p<0.05.
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RCAN 1 protein expressionSyntenic Cross Species Aneuploidy Genetic Screen Links RCAN 1 ExpressionRCAN 1ATPType 2 Diabetes Type 2 diabetesgene expression changesReduced mitochondrial functiontrisomy 21 screening approachgene expression analysisinsulinchromosome 21dysfunctionMice overexpressing RCAN 1Ts 1Rhr miceRCAN 1 methylationDS mouse modelsDp 16 DS mouse linesT 2D islets
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