Quantity and quality of the extracted DNA according to the DNA extraction method.
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posted on 2022-07-13, 17:35 authored by Sandra Jaudou, Mai-Lan Tran, Fabien Vorimore, Patrick Fach, Sabine Delannoy(A) DNA yield (ng.mL-1 culture) as determined with Qubit dsDNA Broad range assay kit, (B) A260/A280 ratio as determined with Nanodrop, (C) A260/A230 ratio as determined with nanodrop, (D) DIN value as determined with Genomic DNA Screentapes.
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take full advantageresulting draft assembliesoverall best methodone major drawbackoften highly fragmentedminimal investment requirementslow error ratesescherichia coli easily span prophagesdiv >< pcontain repeated elementsnext generation sequencingnearly complete genomeslarge structural variationsespecially concerning mgesdna extracted usingmethods performed similarlysubsequent minion runsminion library preparationread sequencing datastec dna yieldlong reads generateddna extraction basedproduce high quantityread sequencingproduce completehigh quantitymges ),large quantitiesmethods allowedbased methodshigh depthsont minionminion longsmall sizeshort readsshiga toxinrepeat regionspathogen characterizationparticularly popularbecome essential
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