Quantitative analyses of rnKCC2b and hsNKCC1 phospho-sites upon staurosporine and NEM treatment in HEKrnKCC2b cells.
Stably transfected HEKrnKCC2b cells were treated with DMSO (control), 8 μM staurosporine or 0.5 mM NEM, respectively, for 15 min. Cell lysates were harvested and subjected to immunoprecipitation (IP) and immunoblot (IB) with indicated antibodies. D, dimeric KCC2; M, monomeric KCC2. Band intensities were quantified with ImageJ software. ***, p < 0.001; **, p < 0.01; Wilcoxon-Mann-Whitney test (n = 6).