Quantification of platelets in patients with chronic Chagas disease.
Platelet levels were measured by platelet counts, using an optical automated hematological counter (Fig 1A) and by the expression of CD41a (Fig 1B) determined by flow cytometry in whole blood samples. Each symbol represents the number of platelets per ml (Fig 1A) or the percentage of CD41a+ cells (Fig 1B). Median values are indicated by the horizontal lines. *P ≤ 0.05 and **p ≤ 0.01 calculated by an ANOVA test followed by a Bonferroni test for multiple comparisons. The oblique line indicates a significant tendency between medians calculated using a test for lineal trends. The full-black symbols represent patients belonging to stages G2 and G3.