Quantification of mitochondrial DNA copy number in RCC1L silencing experiments.

Quantitative PCR (qPCR) analysis of mitochondrial DNA copy number in silenced HEK cells. Silencing of RCC1L^V1 (RNAi 1–3) and RCC1L^V3 (RNAi 4–6) isoforms and combined silencing of RCC1L^V1 and RCC1L^V3 (RNAi 7–8) and all three isoforms (V1+2+3, RNAi, 9–10) in HEK cells. Mock (M) and AllStars dsRNA (S) transfected cells were used as negative controls. Data represent the average of MT-CO1, MT-CYB and 12S rRNA normalised to APP from three independent experiments. See S11 Table for quantitative data in this figure.

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