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Quantification and analysis of the RNAi mediated by synthetic siRNAs targeting over 100 human genes

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posted on 31.12.2011, 07:18 by Scott E. Martin, Tamara L. Jones, Cheryl L. Thomas, Philip L. Lorenzi, Dac A. Nguyen, Timothy Runfola, Michele Gunsior, John N. Weinstein, Paul K. Goldsmith, Eric Lader, Konrad Huppi, Natasha J. Caplen

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Taken from "Multiplexing siRNAs to compress RNAi-based screen size in human cells"

Nucleic Acids Research 2007;35(8):e57-e57.

Published online 28 Mar 2007

PMCID:PMC1885663.

© 2007 The Author(s)

() A ranking of the percentage silencing mediated by 258 siRNAs corresponding to 129 human genes. The percentage silencing was determined by comparing the mRNA levels for each gene following transfection with 50 nM target-specific siRNA to levels found in cells transfected with 50 nM negative-control siRNA. Data is shown as the mean percentage decrease in target mRNA levels observed for four independent parallel transfections (plus SD). Analysis was conducted in HCT-116 (118 siRNAs), MDA-MB-231 (138 siRNAs) and NCI/ADR-RES (two siRNAs). () The percentage decrease in mRNA and protein levels for 19 genes (two siRNAs per gene). mRNA levels were measured 48 h post-transfection, whereas total protein levels were measured 48–96 h post-transfection.

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