ppat.1006558.g003.tif (528.26 kB)

Q-TOF LC/MS analysis of GspB736flag after trypsin, Lys-C, and Glu-C protease digestion.

Download (528.26 kB)
posted on 21.08.2017 by Ravin Seepersaud, David Sychantha, Barbara A. Bensing, Anthony J. Clarke, Paul M. Sullam

Extracted ion chromatograms for all detected glycopeptides derived from GspB736flag secreted from A) a Δgly-nss strain (PS3309) or B) a Δgly-nss Asp2S362A strain (PS3540). Peaks with the same peptide backbone are designated with the same color (green, red, black and blue, respectively) and asterisks denote those containing an O-acetylated glycopeptide. C) Fragmentation spectrum of peak 1, m/z = 484.73 [M+2H]2+ O-glycosylated at two positions. D) Fragmentation spectrum of peak 1*, m/z = 505.74 [M+2H]2+ O-glycosylated at two positions and O-acetylated at one position. O-GlcNAc modifications are designated by a blue square. MS spectra of precursor ions are shown in the insets.