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Protein fractions after chromatography on Ni-NTA affinity chromatography column

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posted on 22.05.2020, 13:11 by Tran Thi Hong, Ton That Huu Dat, Nguyen Phuong Hoa, Tran Thi Kim Dung, Vu Thi Thu Huyen, Le Minh Bui, Nguyen Thi Kim Cuc, Pham Viet Cuong
Protein fractions after chromatography on Ni-NTA affinity chromatography column. (A) SDS-PAGE gel, lane M: marker protein (Thermo); lane 1, 2, 3: total protein eluted with imidazole of 100 mM, 300 mM, 500 mM, respectively. (B) Purity level and amount of protein after chromatography. Different letters indicate significant differences between groups (p < 0.05).

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