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Proposed model for the alkaline pH signaling in N. crassa.

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posted on 24.08.2016, 17:31 authored by Stela Virgilio, Fernanda Barbosa Cupertino, Natália Elisa Bernardes, Fernanda Zanolli Freitas, Agnes Alessandra Sekijima Takeda, Marcos Roberto de Mattos Fontes, Maria Célia Bertolini

(A) The PAC-3 protein contains 621 amino acid residues and has three C2H2 zinc-finger domains encompassing the amino acid from 95 to 183. The NLS sequence is shown between amino acid residues 281 and 304. The black arrow indicates the putative protease-processing site at amino acid 490. (B) N. crassa has the six A. nidulans Pal homologues. External pH signaling may involve the PAL-8, PAL-9, PAL-6 and PAL-3 complex. PAL-1 may act together with PAL-2 and may recruit the PAC-3 protein. PAC-3 undergoes only one proteolytic processing and is likely involved in the activation of the pal-1, pal-2, pal-9, and pac-3 genes and the repression of the pal-6 and pal-8 genes at alkaline conditions. aa, amino acid; ZF, C2H2 zinc finger; NLS, nuclear localization signal.