Fig 2.tif (3.84 MB)

Progressive amyloid formation in human islets during culture is associated with reduced β-cell phospho-PKB levels and proliferation rate.

Download (3.84 MB)
figure
posted on 23.02.2018 by Yun Zhang, Garth L. Warnock, Ziliang Ao, Yoo Jin Park, Nooshin Safikhan, Aziz Ghahary, Lucy Marzban

(A) Paraffin-embedded sections from pre-culture and 7-day cultured human islets with or without amyloid binding dye Congo red (CR; 25 μmol/L) were immunolabelled for insulin, phospho-PKB (p-PKB), and thioflavin S (Thio S). The squares (dashed white lines) correspond to enlarged areas in each image (original magnification: X400; insert: X1000). (B) Immunolabelling for insulin or glucagon and phospho-PKB in amyloid-positive and negative human islets. The percentage of (C) thioflavin S (amyloid)-positive islets and (D) islet amyloid area. (E) Islet phospho-PKB immunofluorescence intensity (IF). The proportion of (F) PCNA-positive (proliferative) β-cells and (G) TUNEL-positive (apoptotic) β-cells in each condition. Results are expressed as mean +/- SEM of five independent studies (25–30 islets per condition in each study). For β-cell phospho-PKB IF intensity, quantifications were performed on a total of 18 amyloid (thio S)-positive 7-day cultured human islets or equal number of CR-treated islets (no or very little amyloid formation). *vs Day 0; #vs corresponding untreated group (P<0.05; one-way ANOVA).

History

Licence

Exports