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Profiling of cell surface receptor phenotype of VZV cultured NK cells.

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posted on 13.06.2019, 17:53 by Tessa Mollie Campbell, Brian Patrick McSharry, Megan Steain, Tiffany Ann Russell, David Carl Tscharke, Jarrod John Kennedy, Barry Slobedman, Allison Abendroth

PBMCs were mock cultured, exposed to VZV, or VZV infected untreated or with 200 U/ml IL-2 for 1 day. NK cells (viable CD3CD56+ cells) were assessed by flow cytometry for cell surface receptor expression (A), and heatmaps show receptor expression as measured by percentage positive with hierarchical clustering for 3 donors (denoted A, B and C) (B). (C) Graph shows fold change over mock in median fluorescence intensity (MFI) for ubiquitously expressed receptors (n = 3). Symbols represent individual donors. Dotted line at y = 1 indicates point of variance from mock. Statistical analysis performed compared to mock. **P < 0.01, ***P < 0.001, ****P < 0.0001 (repeated measures two-way ANOVA with Dunnett’s correction).