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Production and characterisation of nanoparticles with bound oligonucleotides.

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posted on 17.09.2020, 17:30 by Nayab Fatima, Radka Gromnicova, Jane Loughlin, Basil Sharrack, David Male

(A) EMSA of exchange reaction (1–4 days) probed with a biotinylated oligonucleotide complementary to the ssDNA oligonucleotide attached to the NPs. (B) FPLC trace of reaction products of exchange reaction with 20nt thiol-C6-ssDNA, at 48hrs. y-axis: pressure and absorption at 280nm. x-axis: time (0–60 minutes) and fraction number (1–41). (C) EMSA of FPLC fractions (15–29) of NP-DNA. The numbers 15–29 correspond to the fraction numbers shown in part Fig1B.

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