Predicted (Red) versus actual (Black) solvent accessible surface area (ASA) of an RNA along with its structure colored coded for accessibility.

Results of a corresponding random sequence (randomly shuffled from the original sequence) were shown in Blue. (A) Lysine-tRNA from E. coli (PDB: 5IB8, chain: 1K), (B) 5S RNA from Thermus thermophilus (PDB: 5IB8, chain: 16) and (C) E coli’s mRNA in the same complex (PDB: 5IB8, chain: 4L) Experimental structures were color-coded according to the relative ASA: 5+ for Red, 4 for Orange, 3 for Yellow, 2 for Green and 1 for Blue.