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Pom-treatment of both PEL and T cells produces higher T-cell activation than treatment of either alone.

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posted on 07.01.2021, 19:26 by Prabha Shrestha, David A. Davis, Hannah K. Jaeger, Alexandra Stream, Ashley I. Aisabor, Robert Yarchoan

(A) IL2-Jurkat cells were grown in the absence or presence of 1μM Pom. After 48 hours, Pom was washed out and they were incubated for 6 hours with or without control PEL cells in the absence or presence of various concentrations of anti-CD3 antibody. Activations of ctrl or Pom-treated IL2-Jurkat cells in the absence or presence of ctrl BCBL-1 (left) or JSC-1 (right) cells are expressed as average RLU from 3 separate experiments. (B) Both PEL cells and IL2-Jurkat cells were cultured in the absence or presence of Pom. After 48 hours, cells were washed with PBS to remove Pom and Jurkat T-cell activation assay was performed with various concentrations of anti-CD3 Ab. Activations of ctrl or 1μM Pom-treated Jurkat cells by ctrl or Pom-treated BCBL-1 (left) or JSC-1 (right) cells are expressed as average RLU from 3 separate experiments.

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