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Polyacrylamide gel electrophoresis (PAGE) showing successful parallel synthesis of genes along with negative controls

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posted on 31.12.2011, 07:18 by David S. Kong, Peter A. Carr, Lu Chen, Shuguang Zhang, Joseph M. Jacobson

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Taken from "Parallel gene synthesis in a microfluidic device"

Nucleic Acids Research 2007;35(8):e61-e61.

Published online 2 Apr 2007

PMCID:PMC1885655.

© 2007 The Author(s)

In the presence of construction oligos, DNA constructs GFP and dsRed (993 and 733 bp, respectively; Figure 4A) and OR128-1 and ble (942 and 461 bp, respectively; Figure 4B) are synthesized and amplified. Without construction oligos, no product bands are generated. Molecular weight markers are shown (M) with 500, 750 and 1000 bp positions indicated.

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