Podocytes in Tsc2Δpodocyte mice were excreted in urine from glomeruli with progression of renal functional decline.
(A) Immunofluorescence staining of podocin and synaptopodin showed decreased signal intensity in Tsc2Δpodocyte mice (arrows). (B, top) The number of podocytes was decreased in the glomeruli of Tsc2Δpodocyte mice. Representative images of WT1 positive (red) and Hoechst 33342-positive (blue) podocytes are shown. (B, bottom) The graph shows the average number of podocytes per glomerulus in each group. Each dot represents the mean number of WT1-positive cells in about 20 glomeruli from the indicated mouse, and horizontal lines represent the mean number of WT1-positive cells in each group. Tsc2Δpodocyte mice had fewer WT1-positive podocytes per glomerulus compared with age-matched controls. *P < 0.05, **P < 0.01 compared with age-matched controls. (C) Western blot analysis of abundance of WT1 in urine from Tsc2Δpodocyte mice. Loaded samples contained equal amounts of creatinine. WT1 gives a band at 52 kDa (arrow).