Plasmid and large insert vectors used in ISSI
figure
posted on 2011-12-30, 15:32 authored by Felix Dafhnis-Calas, Zhengyao Xu, Steve Haines, Sunir K. Malla, Margaret C. M. Smith, William R. A. BrownCopyright information:
Taken from "Iterative assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase"
Nucleic Acids Research 2005;33(22):e189-e189.
Published online 15 Dec 2005
PMCID:PMC1316120.
© The Author 2005. Published by Oxford University Press. All rights reserved
() Plasmid Hytk used in the ISSI sequence shown in . () Plasmid BSR used in the ISSI sequence shown in . () BAC vector φC31BAC CCAGHyTk. This vector differs from the others in that it includes a promoter upstream of the selectable marker. We originally expected that this modification would improve the efficiency of the integration compared with a promoterless vector however comparisons revealed no difference in the respective efficiencies. This vector was used in the experiments shown in . Promoterless BAC and PAC equivalents have been engineered (Supplementary Data). () PAC vector φC31PAC BSR. This vector was used as the vector for the human X chromosome alphoid DNA in the experiment shown in . A BAC equivalent has been engineered (Supplementary Data).