Plaque morphology of ΔNS2-L19F-4M and ΔNS2 before and after 10 rounds of serial passage.
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posted on 2024-04-09, 17:56 authored by Judith Alamares-Sapuay, Michael Kishko, Charles Lai, Mark Parrington, Simon Delagrave, Richard Herbert, Ashley Castens, Joanna Swerczek, Cindy Luongo, Lijuan Yang, Peter L. Collins, Ursula J. Buchholz, Linong ZhangImages of representative wells from titrations of (A) original ΔNS2-L19F-4M material and (B) harvest from the 10th round of serial passage. Plaques were grown under overlay for 7 days at 32°C.
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strain a2 increasedrespiratory syncytial virusns2 /&# 916licensed pediatric vaccineleading viral causehuman airway epithelialafrican green monkeys37 ° c32 ° c25 ° cleading vaccine candidatehighly immunogenic prediv >< p4 ° c2 clinical trialsimilar growth kineticspeak titer similarclinical trialpeak titergrowth kineticsδns2 candidatevitro serial passageresults suggestpresent studymissense mutationintranasal immunizationgenetically stablefold higherconfers temperature28 days10 rounds
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