Phylogenetic analysis of Chionaster nivalis and related Agaricomycetes, Wallemiomycetes, and Ustilaginomycetes.
A maximum-likelihood phylogeny generated using a concatenation of the internal transcribed spacer (ITS) and D1-D2 region of the LSU rRNA gene using the SYM+I+G4 nucleotide substitution model following the removal of the 20% fastest evolving sites, as inferred in IQ-Tree, to limit long branch attraction. The included taxa are similar to those included in Fig 2 with the addition of Wallemiomycetes, Ustilaginomycetes, and additional Agaricomycetes which represent more distant outgroups for the Tremellomycetes. All species are represented by both the ITS and the D1-D2 with the exception of certain uncultured environmental fungi and C. nivalis 97C (see S1 Table). Statistical support is shown at each node and was generated from 1000 ultrafast bootstraps (UFB), 1000 non-parametric bootstraps (NPB), and Bayesian posterior probabilities (PP). Values above 95 UFB, 95 NPB, and 0.99 PP are indicated with black circles whereas values below 75 UFB, 75 NPB, and 0.95 PP are not shown. Chionaster nivalis is highlighted in black and the five recognized Tremellomycete orders have been outlined in grey. For clarity, clades comprising over three representatives of exclusively the same genus or representing the Tremellales, Trichosporonales, and Holtermanniales were collapsed and are shown as wedges. See S1 Table and Fig 2 for a list of taxa and their respective accession numbers.