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Visualisation of lyso-PChol488 (20μM) co-stained with antibodies recognising replication intermediates (dsRNA).

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posted on 30.04.2018, 17:41 by Susann Liebscher, Rebecca L. Ambrose, Turgut E. Aktepe, Andrea Mikulasova, Julia E. Prier, Leah K. Gillespie, Adam J. Lopez-Denman, Thusitha W. T. Rupasinghe, Dedreia Tull, Malcolm J. McConville, Jason M. Mackenzie

Panels i and ii, mock- and WNVKUN-infected Vero cells stained with anti-dsRNA antibodies and visualised with AF594. Panels iii-vii, higher magnification of the insert identified with the white box in panel ii. Panels vii and viii, mock- and WNVKUN-infected Vero cells stained with anti-dsRNA antibodies and visualised with AF594 and incubated with 20μM lyso-PChol488. Panels ix-xii, higher magnification of the insert identified with the white box in panel viii. In all case the cells were counterstained with Hoechst 33342 to visualise nuclei and the white arrows indicate areas of colocalisation.

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