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Percentage of foci generation (Foci %) in the Balb/c 3T3 cellular transformation assay.

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posted on 10.01.2020, 18:45 by Emilio Rojas, Monica Martinez-Pacheco, Maria Alexandra Rodriguez-Sastre, Paulina Ramos-Espinosa, Mahara Valverde

(A) Cellular transformation foci analysis on day 13 of the assay from cultures treated with the miR-222 precursor molecule as initiator stimulus and the known TPA promoter (pre-miR-222/TPA), with the known initiator MNNG and the miR-222 precursor molecule as promoter stimulus (MNNG/pre-miR-222) or with the miR-222 precursor molecule as initiator and promoter stimulus (pre-miR-222/pre-miR-222), compared to the control condition (Control/Control). (RCFEs were divided over the Control/Control condition of the respective day and multiplied by 100) N = 2, mean ± SE, one-way ANOVA F (5,6) = 49043 *p<0.0001, Tukey post-hoc comparisons of the groups 95% CI. (B) Relative colony formation efficiency (RCFE) of Balb/c 3T3 cells of day 13, for cells treated with the pre-miR-222 molecule as an initiator and/or promoter stimulus. N = 2, mean±SE, two-tailed un paired t-test, *p<0.05.

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