Peptidoglycan–polysaccharide (PG-PS)-induced increase in intracellular calcium concentration (Ca) is inhibited in CD14 knockout cells
figure
posted on 2011-12-31, 04:37 authored by Xiangli Li, Blair U Bradford, Frederick Dalldorf, Sanna M Goyert, Stephen A Stimpson, Ronald G Thurman, Sergei S MakarovCopyright information:
Taken from "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls"
Arthritis Research & Therapy 2004;6(3):R273-R281.
Published online 27 Apr 2004
PMCID:PMC416450.
Copyright © 2004 Li et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
The levels of [Ca]in spleen macrophages were evaluated by using fura-2, a fluorescent [Ca] indicator. Kinetics of PG-PS-induced [Ca](average from six to eight cells). Representative data from two experiments are shown. The average maximal increase in [Ca]shown as a function of PG-PS concentration. Data represent means ± standard error of the mean for 12–34 cells from two independent experiments. The significance of the difference between the groups was calculated using one-way analysis of variance with Scheffe's test. *< 0.05 versus wild-type.