Peptidoglycan–polysaccharide (PG-PS)-induced activation of nuclear factor-κB (NF-κB) is inhibited in CD14 knockout spleen macrophages

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posted on 2011-12-31, 04:37 authored by Xiangli Li, Blair U Bradford, Frederick Dalldorf, Sanna M Goyert, Stephen A Stimpson, Ronald G Thurman, Sergei S Makarov

Copyright information:

Taken from "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls"

Arthritis Research & Therapy 2004;6(3):R273-R281.

Published online 27 Apr 2004

PMCID:PMC416450.

Copyright © 2004 Li et al.; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

Spleen macrophages were stimulated for 30 min with PG-PS at a concentration of 20 μg/ml. The left column shows immunostaining of the p65 (RelA) subunit of NF-κB, and the right column shows counterstaining of nuclei with Hoechst 33342. The rows show the following: top – unstimulated cells from wild-type mice; middle – PG-PS stimulated cells from wild-type mice; and bottom row – PG-PS-stimulated cells from CD14 knockout mice. Representative data from three independent experiments are shown.