pone.0177192.g013.tif (2.95 MB)

PTPμ expression in developing and adult mouse vasculature.

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posted on 25.05.2017, 17:34 by Keiko Takahashi, Rachel Kim, Colette Lauhan, Yuna Park, Nghiep G. Nguyen, Dietmar Vestweber, Melissa G. Dominguez, David M. Valenzuela, Andrew J. Murphy, George D. Yancopoulos, Nicholas W. Gale, Takamune Takahashi

(A though F) PTPμ was visualized in developing and adult mice kidneys by immunofluorescence staining using anti-PTPμ mouse monoclonal antibody. PTPμ immunoreactivity is relatively limited in developing kidneys at E15.5 (panel A) and P0 (panel B) stages. Weak immunoreactivity is observed in large renal arterial vessels (A) in P0 kidney. At P3 (panel C) and P7 (panel D), PTPμ is expressed in maturing glomeruli (G) and large renal arterial vessels including renal artery and its branch (an insert in panel D). In adult kidney (panels E and F), PTPμ is expressed in the endothelium of large renal arteries (renal artery to arcuate artery) (A) and glomeruli (G). (G) Endothelial localization (yellow arrows) of PTPμ in glomerulus was confirmed by co-immunostaining of PTPμ and CD31. A, arterial vessel; G, glomerulus; V, vein. Scale bar, 60 μm in A, B, C, D, E, and G; 30 μm in F.