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PMN expression of intracellular cytokines after stimulation with different Salmonella strains.

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posted on 14.08.2019, 17:47 by Rosangela Salerno-Goncalves, Darpan Kayastha, Alessio Fasano, Myron M. Levine, Marcelo B. Sztein

3-D organotypic models were exposed, or not, to either Salmonella enterica serovar Paratyphi A (PA), Paratyphi B (PB), or Typhi (ST) strains. After 4 hours, supernatants were collected and used to stimulate dextran-500-purified PMN. (A) After 4 hours of stimulation, PMN were surface and intracellular stained, and the levels of IL-6, IL-8, TNF-α and CCL3 intracellular cytokines measured by flow cytometry. PMN were gated based on their scatter characteristics and specific lineage differentiation markers: CD45+CD163-CD66c+. Numbers correspond to the % positive cells, followed by mean fluorescence intensity (MFI) in parenthesis (x-axis). (B) Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. The data represent 2 individual experiments with up to 3 replicates in each experiment. Horizontal lines represent significant differences (P<0.05) between the indicated culture conditions.