PDF rhythmic accumulation is similar in males and females (related to Fig 1).

(A) Representative confocal images of control (w¹¹¹⁸) (top) and experimental (Pdf⁰¹) (bottom) male and female flies stained with the PDF antibody. (B) Scatterplots of PDF staining intensities of the s-LN_v dorsal projections of both male and female flies plotted at different time points over a 24-h cycle on DD day 3. Each dot represents the mean PDF intensity value averaged over both hemispheres of one brain. The cyan and pink lines are the best-fit cosine curves from the parameters that were extracted via COSINOR analysis. See Table 2 for more details. (C) Amplitude values of PDF oscillation obtained from COSINOR curve fits are plotted for male and female flies. The error bars are 95% CI values calculated from the standard error obtained from COSINOR analysis. The overlapping error bars indicate that the amplitude values of males and females are not significantly different. n > 7 brain samples/time point. (D) Nuclear RFP intensity values are plotted for the sLN_vs of Pdf > nls-mCherry males (n = 14 brains) and females (n = 10 brains). The data used in S1 Fig can be found at https://doi.org/10.6084/m9.figshare.28601366.v1.

(EPS)