pone.0215188.g006.tif (480.15 kB)

PDE1 inhibition reduces palmitate-induced apoptosis and stimulates CREB phosphorylation in INS-1 cells.

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posted on 23.08.2019 by Evan P. S. Pratt, Kyle E. Harvey, Amy E. Salyer, Gregory H. Hockerman

A) Incubation of INS-1 cells with palmitate for 12h significantly elevates caspase-3/7 activation (*, P < 0.05 compared to BSA; Student’s unpaired t-test). Data shown are average ± SE from six independent experiments. B) The PDE1 inhibitor 8MM-IBMX (100 μM) and pan PDE inhibitor IBMX (100 μM) significantly decrease the level of caspase-3/7 activation induced by palmitate toxicity (**, P < 0.01, *, P < 0.05 compared to palmitate; One-way ANOVA, Tukey post-hoc test). Each experiment was normalized to the level of palmitate toxicity. Data shown are average ± SE from 4–6 independent experiments. C) The PDE1 inhibitor 8MM-IBMX (100 μM) and pan PDE inhibitor IBMX (100 μM) significantly stimulate CREB phosphorylation above basal levels (****, P < 0.0001, *, P < 0.05 compared to basal; ##, P < 0.01, #, P < 0.05 compared to IBMX; One-way ANOVA, Tukey post-hoc test). Data shown are average ± SE from four independent experiments.

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