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PCR stop assay.

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posted on 18.10.2018, 17:47 authored by Shiv Prakash Verma, Parimal Das

Oligo sequence of IGFN1 wild type(IGFN1-W), mutant type(IGFN1-M) and partially complementary(IGFN1-rev) oligo used in PCR stop assay, overlapping complementary last G-repeat sequence is underlined (A); Gel picture showing the inhibition of Taq DNA polymerase extension leading to the PCR stop at 0, 1, 2, 3, 4, 5 μM PDS concentration in IGFN1-W (B) and IGFN1-M oligo (C); PCR stoppage by G-quadruplex stabilization in presence of 4 μM PDS was rescued in presence of different molar concentration (0, 1, 2, 3, 4, 5 times) of IGFN1-C antisense oligonucleotide (ASO) with respect to molar concentration of IGFN1-W (D).

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