PCA of the RNA-seq samples (A), heatmap showing altered gene expression in male-biased and female-biased clusters (B) and key players in sex determination and TSR: Key players adapted from previous studies, showing significant expression changes (C) [32–35,50].
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posted on 2025-04-30, 17:46 authored by Jigang Lu, Siqi Huang, Shicen Wei, Jiangbo Cheng, Wei Li, Yueyue Fei, Jihui Yang, Ruiqin Hu, Songqian Huang, Wanying Zhai, Zhichao Wu, Mingli Liu, Qianghua Xu, Peng Hu, Liangbiao ChenPCA of the RNA-seq samples (A), heatmap showing altered gene expression in male-biased and female-biased clusters (B) and key players in sex determination and TSR: Key players adapted from previous studies, showing significant expression changes (C) [32–35,50].
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still largely unknownpreviously unrecognized intronobserved significant upregulationnile tilapia (<enabling nuclear translocationbiochemical studies confirmedmrna alternative splicinginfluences sex determinationdependent sex reversalkdm6bb significantly impactsoreochromis niloticus promoting genes (<mechanisms translating temperaturediv >< ph3k27 methylation levelh3k27 demethylase kdm6bbalternative inclusionkdm6bb wt1a gsdf foxl3 dmrt1 amh subcellular localizationsexual differentiationsecondary effectsproven challengingpromoter regionsmethylation changeskey factorhigh temperaturegene deletionfindings refuteelevated temperaturesdetermining signalcritical rolecombined transcriptomic
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