Fig 1.TIF (485.2 kB)
P2X7R and pannexin 1 channels form molecular complexes in bone cells.
figure
posted on 2016-05-09, 06:06 authored by Zeynep Seref-Ferlengez, Stephanie Maung, Mitchell B. Schaffler, David C. Spray, Sylvia O. Suadicani, Mia M. ThiP2X7R and Panx1 protein-protein interaction was assessed by immunoprecipitating lysates from long bone tissue, MLO-Y4 osteocytes and MOB-C osteoblasts with P2X7R antibody followed by Western blotting with Panx1 and P2X7R antibodies. “+” lanes represent samples with P2X7R antibody and “-” lanes represent samples with no antibody. Note: Both MLO-Y4 and MOB-C express two bands of P2X7R [functional glycosylated form at ~79kD and a splice variant at ~ 56kD [56]]. Bands corresponding to IgG heavy chain (~50 kD) are indicated on the blot with P2X7R antibody.
History
Usage metrics
Categories
Keywords
T 1D causesP 2Y 4 R expressionosteocyte cell linesWestern Blot analysisP 2Rsglucose levelsP 2X Complex Impairs Bone MechanosignalingreleaseP 2X P 2Y 2 R25 mM glucosebone cellsDiabetes Type 1 diabetesP 2RATPHigh glucose effectsfunctionHigh Glucose Levels AssociatedPanx 1 channelsDiabetic C 57BL Akita miceP 2R agonists
