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P1 and P2 reduce IL-10 secretion in U937 cells stimulated by LPS.

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posted on 21.04.2016, 06:02 by Guoying Ni, Shu Chen, Yuedong Yang, Scott F. Cummins, Jian Zhan, Zhixiu Li, Bin Zhu, Kate Mounsey, Shelley Walton, Ming Q. Wei, Yuejian Wang, Yaoqi Zhou, Tianfang Wang, Xiaosong Liu

Supernatants were measured in the presence of IL-10 by ELISA. The concentration of LPS is 4×10−3 μM: (A) 3×105 human U937 were either left unstimulated (UN) or stimulated with LPS, LPS+0.3 μM of anti-IL10 (aIL10), LPS+0.1 μM of aIL10R, LPS+P1, 2, 3 and 4 at 4.5, 4.1, 5.6, 4.2 μM overnight, respectively. (B) 1×105 of U937 cells were treated with LPS and different concentration of P1 overnight. (C) 1×105 of U937 cells were treated with LPS and different concentration of P2 overnight. (D) 1×105 of U937 cells were unstimulated or stimulated with LPS, LPS+aIL10, LPS+aIL10R, LPS+P1 and LPS+P2 for 4, 12, 24 and 48 hours.

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