Ovol1 competes with c-Myb for DNA binding and represses c-Myb-activated promoter activity
figure
posted on 2011-12-31, 06:30 authored by Mahalakshmi Nair, Virginia Bilanchone, Kori Ortt, Satrajit Sinha, Xing DaiCopyright information:
Taken from "Ovol1 represses its own transcription by competing with transcription activator c-Myb and by recruiting histone deacetylase activity"
Nucleic Acids Research 2007;35(5):1687-1697.
Published online 20 Feb 2007
PMCID:PMC1865076.
© 2007 The Author(s).
() Results of EMSA assays showing that Ovol1 binds to c-Myb cognate site but not (for oligonucleotide sequences, see Materials and Methods). ‘-’, no protein. ▹ represents increasing concentrations (216–650 nM) of recombinant Ovol1 protein. () Ovol1 competes with c-Myb for interaction with . Lane 1, free probe; lane 2, 0.8 μg of c-Myb bacterial extract; lanes 3–6, 0.8 μg c-Myb extract and increasing concentrations (31–188 nM) of recombinant Ovol1 protein; lane 7, 0.8 μg c-Myb extract + recombinant Ovol1 (188 nM) + anti-Ovol1 antibody. Oval, arrowhead and arrow represent c-Myb–DNA, Ovol1–DNA and Ovol1–DNA–antibody complexes, respectively. () Results of ChIP assays showing that Ovol1 competes with c-Myb for binding to the endogenous promoter. Equivalent levels of Ovol1 and ZnFC2A proteins were expressed (data not shown). () c-Myb activates the promoter in an Ovol1-binding site-dependent manner. () Ovol1 represses c-Myb-activated promoter activity in a DNA binding-dependent manner.