Overview of the 6-plex mycotoxin immunoassay: Mycotoxin mAb coupled paramagnetic beads and a mixture of mycotoxin specific reporter molecules (mycotoxins coupled to R-phycoerythrin) are added to a diluted beer sample.
Competition occurs between the free mycotoxins present in the beer and the added mycotoxin-reporter molecules for antibody interaction on the beads. Next, the beads are captured at the bottom of the well by magnetic force. Remaining, non-interacting, assay components are removed by washing. Beads are then measured using a red light source for mycotoxin assay classification and a green light source for quantification of the reporter signal.