Overview of differentially regulated genes mediated by attenuated and virulent strains.

A) The relative expression levels of immune-related genes in the blood were calculated using the 2^-ΔΔCt method. The error bars represent standard errors of the mean. Statistically significant differences induced by attenuated and virulent strains between the mean gene expression levels were determined by one-way ANOVA. *P < 0.05, **P < 0.01 or ***P < 0.001 indicate the level of statistical significance of differences between the different groups. B) The frequencies of differentially regulated genes in all selected tissues and specific tissues are shown as a Rader map (left and right).