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Overlap of OTUs generated using Uclust between/among technical replicates at different sequencing depth.

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posted on 2017-04-28, 17:28 authored by Chongqing Wen, Liyou Wu, Yujia Qin, Joy D. Van Nostrand, Daliang Ning, Bo Sun, Kai Xue, Feifei Liu, Ye Deng, Yuting Liang, Jizhong Zhou

At a sequencing depth of about 30,000 reads, overlap of both two and three replicates was approaching a plateau when singletons were not removed.

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Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform
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treatment effectsamplicon sequencing16 S rRNA gene ampliconsV 4 region16 S rRNA geneUMIsequence abundanceMiSeq18 soil samplesgene amplicon sequencingPCRreplicatereproducibilityDetrended correspondence analysisOTU sequence abundanceOTU overlap

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