Overall 17DD-YF memory-related biomarker signatures at distinct time-points after primary vaccination.

The phenotypic/functional biomarker signatures were built taking the proportion of subjects above the cut-off edges defined for each attribute, calculated as the median index value (17DD-YF/Control) for the study population. Diagrams were constructed for all study groups to calculate the proportion (%) of volunteers above the median cut-off indices for each biomarker (gray-shaded spots). (A) The PV(day30-45) group was used to construct the memory-related phenotypic and functional biomarker signatures and draw the reference curves, used for comparative analysis amongst the study groups, (B) NV(day0), (C) PV(year1-9) and (D) PV(year10-11). Hatched cells represent unavailable results. Data mining was carried out as proposed previously by Luiza-Silva et al., (2011), selecting from the PV(day30-45) reference curves, those biomarkers with more than 50% of volunteers above the cut-off index (surrounded by dashed rectangles). Comparative analysis amongst the study groups were carried out considering only the selected set of relevant biomarkers from the phenotypic and functional reference curves. Substantial change in the set of relevant biomarkers were highlighted by (*) when the proportion of subjects above the cut-off fell below 50%. The common set of relevant biomarkers on each study group was underscored in bold font.