Figure S1 - Over-Expression of miR-106b Promotes Cell Migration and Metastasis in Hepatocellular Carcinoma by Activating Epithelial-Mesenchymal Transition Process
QPCR analysis in cell lines and clinical sample. (A) miR-106b expression in HCC cell lines is higher than immortalized normal hepatocyte cell line MIHA. (B) Higher miR-106b expression was observed in metastatic cell lines than the primary tumor cell lines. (C) miR-21 and (D) miR-106b expression were analyzed in HCC clinical sample by QPCR. Expression of miR-21 and miR-106b was significantly higher in HCC tumor tissue. (E) Successful knock-down of miR-106b expression was attained within 4 days after transfection. (F) Over-expression of miR-106b in PLC-PT, Huh7 and Hep3B cell lines can be attained by lentiviral transduction by comparing with the empty vector control.