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Figure S1 - Over-Expression of miR-106b Promotes Cell Migration and Metastasis in Hepatocellular Carcinoma by Activating Epithelial-Mesenchymal Transition Process

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posted on 2013-03-07, 08:34 authored by Wing Lung Yau, Colin Siu Chi Lam, Lui Ng, Ariel Ka Man Chow, Sylvia Tsz Ching Chan, Jacky Yu Ki Chan, Jana Yim Hung Wo, Kevin Tak Pan Ng, Kwan Man, Ronnie Tung Ping Poon, Roberta Wen Chi Pang

QPCR analysis in cell lines and clinical sample. (A) miR-106b expression in HCC cell lines is higher than immortalized normal hepatocyte cell line MIHA. (B) Higher miR-106b expression was observed in metastatic cell lines than the primary tumor cell lines. (C) miR-21 and (D) miR-106b expression were analyzed in HCC clinical sample by QPCR. Expression of miR-21 and miR-106b was significantly higher in HCC tumor tissue. (E) Successful knock-down of miR-106b expression was attained within 4 days after transfection. (F) Over-expression of miR-106b in PLC-PT, Huh7 and Hep3B cell lines can be attained by lentiviral transduction by comparing with the empty vector control.