Origin-by-origin assessment of the SortSeq data generated by the FHA-ARS-regulated origin groups.

<p><b>(A)</b> Ratio of ARS activity in <i>fkh1-R80A</i> cells to <i>FKH1</i> cells (y-axis) for indicated origins (xaxis). FHA-ARS-dependent origins indicated by black bars while FHA-ARS-independent origins indicated by grey bars. <b>(B)</b> Close-up of S-phase copy number scans for FHA-ARS-regulated origins in <i>FKH1</i> (black) and <i>fkh1-R80A</i> (red) cells. <b>(C)</b> The <i>fkh1-R80A</i>/<i>FKH1</i> S-phase copy number ratio determined for each FHA-ARS regulated origin color coded based on its FHA-SORT assigned status. FHA-ARS-regulated origins were defined initially based on ORC-origin binding mechanisms and subsequently parsed by ARS activity. Neither <i>ARS609</i> nor <i>ARS224</i>, each an FHA-ARS-dependent origin, qualified as FHA-SORT-positive. Note that neither origin is active even in <i>FKH1</i> cells on the chromosome. Thus ORC-origin binding mechanisms might not play a major role in their chromosomal activity, yet might have a large impact on origin activity in a more isolated situation, such as on a plasmid.</p> <p>(TIFF)</p>