Origin-by-origin assessment of the SortSeq data generated by the FHA-ARS-regulated origin groups.

(A) Ratio of ARS activity in fkh1-R80A cells to FKH1 cells (y-axis) for indicated origins (xaxis). FHA-ARS-dependent origins indicated by black bars while FHA-ARS-independent origins indicated by grey bars. (B) Close-up of S-phase copy number scans for FHA-ARS-regulated origins in FKH1 (black) and fkh1-R80A (red) cells. (C) The fkh1-R80A/FKH1 S-phase copy number ratio determined for each FHA-ARS regulated origin color coded based on its FHA-SORT assigned status. FHA-ARS-regulated origins were defined initially based on ORC-origin binding mechanisms and subsequently parsed by ARS activity. Neither ARS609 nor ARS224, each an FHA-ARS-dependent origin, qualified as FHA-SORT-positive. Note that neither origin is active even in FKH1 cells on the chromosome. Thus ORC-origin binding mechanisms might not play a major role in their chromosomal activity, yet might have a large impact on origin activity in a more isolated situation, such as on a plasmid.

(TIFF)