OVA-Induced esophageal epithelial TSLP induction is dependent upon NF-kB signaling and inhibited by budesonide in vitro.
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A) differentiated EPC2-hTERT cells were pre-incubated with Bay-11 for 1hr prior to stimulation with OVA. TSLP in culture supernatant was assayed after 24 hrs. B) Differentiated EPC2-hTERT cells were stimulated with Ova in the presence or absence of budesonide (1uM) or DMSO (vehicle) for 24hrs.TSLP in culture supernatant was assayed using ELISA. ***p<0.001, ****p<0.0001, NS = not significant.