Nutlin-3 (an Mdm2 inhibitor) increases G0/G1 arrest induced by alcohol exposure.
A) MCF-7 cells that were pretreated with 0 or 1 μM of Nutlin-3 for 1 hour were exposed to alcohol (0 or 0.8% v/v) for 24 hours. Cell cycle arrest was measured by FACS analysis. B) The graph depicts the average percentage of cells (± S.E.) in G0/G1, S, and G2/M phase (*p<0.05; **p<0.01) from three replicate experiments. C) p53, p21, Bax, p-H2AX, and H2AX protein expression were determined by Western blot analysis of cells that were pretreated with 0 or 1 μM of Nutlin-3 for 1 hour and then treated with alcohol (0, 0.2%, or 0.4% v/v) for 6 hours.