Normalized S-phase copy number scans across yeast centromeres.

https://doi.org/10.1371/journal.pgen.1011366.s004
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posted on 2024-08-05, 17:34 authored by Timothy Hoggard, Erika Chacin, Allison J. Hollatz, Christoph F. Kurat, Catherine A. Fox

<p>Based on the stringent definition of Cen-associated origins used here, CEN4, CEN6, CEN7, and CEN15 do not contain CEN-associated origins per our definition. Copy numbers are presented as means and 95% confidence intervals from three <i>FKH1</i> (black) and two <i>fkh1-R80A</i> (red) replicates, as discussed in the main text.</p> <p>(TIFF)</p>

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positioned nucleosome array nucleosome depleted region mnaseseq data generated either chromosomal replication current models posit saccharomyces cerevisiae </compare genome replication ≈ 100 origins transcription factor fkh1 fkh1 acts directly proliferating cell populations phase sortseq experiments fkh sites ).dna binding functions div >< p fha domain promoted fha domain controlled origins ’ activity early origins ).distinct chromatin architecture regulated origin activity fha domain enhances chromatin architecture fha domain distinct effects differentially regulated fkh1 </yeast genome r80a </genome scale domain promotes conserved fha yeast origins early origins phase chromatin chromatin state chromatin organization functional fkh1 fkh1 uses fkh1 contains temporal landscape small subset proliferating cells poorly understood phase event origin interactions origin groups origin cycle origin binding mutant cells binding module ask whether

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