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New experiments to confirm results.

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posted on 2024-01-17, 18:34 authored by Rubén González, Marie-Anne Félix

(A) Activation of the pals-5p::GFP reporter upon OrV infection of animals on different single CeMbio strains and the whole CeMbio community. (B) Repetition of experiment shown in Fig 6, but only evaluating the activation of the pals-5p::GFP reporter. (C) Repetition of experiment shown in Fig 6, using a transfer of an agar chunk after 2 generations (detailed in methods). (D) Repetition of experiments, testing drh-1 animals, shown in Fig 8D. (E) Susceptibility to viral infection on Lelliottia JUb276 of animals carrying a natural deletion allele of drh-1. Data are presented as mean ± standard error. Black symbols indicate the significance of the difference between the labeled bacteria and the Escherichia OP50 reference, while red symbols indicate the significance of differences between genotypes: *** P < 0.001; ** P < 0.01; * 0.01 < P < 0.05; P values higher than 0.05 are labeled as “ns”. Significance was calculated using an analysis of variance with bacteria as a factor (Panels A,C,D), a general linear model where the factors were bacteria and generations (Panel D), or a general linear model where the factors were bacteria and host genotype (Panel E). In both cases Tukey contrasts were used for post hoc analyses.

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