Neutrophil infiltration to the liver is attenuated in mice with reduced gut-bacterial load.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
(a-c) Liver expression of neutrophil associated genes E-selectin, Ly6g and Mpo were measured by qPCR and normalized to 18S. (e) MPO immunohistochemical staining to detect neutrophils in the liver parenchyma revealed an increased number of neutrophils/area in alcohol-fed mice compared to pair-feds and reduced gut bacterial load led to fewer infiltrating neutrophils. Interestingly, antibiotics also cause a decreased trend in hepatic neutrophils at baseline in pair-fed mice (quantified in (d)). * p<0.05 by Mann-Whitney test.