Natural <i>N</i>. <i>vectensis</i> populations are associated with specific microbiota.

<p>(A) Sampling sites map. The base layer was obtained at <a href="https://www.diva-gis.org/Data" target="_blank">https://www.diva-gis.org/Data</a>. (B) PCoA (based on Jaccard metric, sampling depth = 5,000) illustrating similarity of bacterial communities based on geographic location of the March-field samples; (C) beta-diversity distance box plots within and between geographic locations, differences were tested through Mann–Whitney U-test (*** = <i>p</i> ≤ 0.001); (D) alpha-diversity comparisons between geographic locations (max rarefaction depth = 5,000, num. steps = 10), differences were tested through Kruskal–Wallis test followed by Dunn’s post hoc comparisons (H = 12.63, * = <i>p</i> ≤ 0.05, ** = <i>p</i> ≤ 0.01); (E) relative abundance of main bacterial groups among different geographic locations. NS (Nova Scotia), ME (Maine), NH (New Hampshire), MA (Massachusetts), NC (North Carolina). Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.3001726#pbio.3001726.s011" target="_blank">S1 Data</a>.</p>