pone.0274958.s006.tif (1008.72 kB)
Native gel electrophoresis of PROM1 clones s8 and s8(-Ex19).
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posted on 2022-10-10, 17:30 authored by Fatimah Matalkah, Scott Rhodes, Visvanathan Ramamurthy, Peter StoilovThe proteins were resolved by native blue electrophoresis, transferred to PVDF membranes, and probed with mAB 13A4 and anti-Flag antibodies as indicated. Lanes containing the size standard were cut from the membranes after the transfer and stained with Ponceau S. The size standard lanes and probed membranes were imaged on Typhoon Phosphorimager (GE Healthcare).
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specific alternative exonsignificantly reduced duemain reagent usedalternative exon restoredthird extracellular domainmajor prom1 isoformphotoreceptor specific isoformmab 13a4 recognizesdiv >< pmouse prom1 proteindeletion mutagenesis demonstratedmab 13a4extracellular domainsprom1 structureprom1 several formsretinal degenerationprimary ciliaphotoreceptor cellsinterpreting tissueanimal models
