NXF2 co-localizes with the components of RNA granules in dendrites of primary neurons
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Taken from "NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins"
Nucleic Acids Research 2007;35(8):2513-2521.
Published online 1 Apr 2007
© 2007 The Author(s)() GFP-NXF2-expressing neurons were processed for hybridization with Cy3-labeled oligo(dT) probe (red). The insert in the merged image is a blow-up of the region of the image indicated. Scale bars, 10 μm. () Left: GFP-NXF2-expressing neurons were processed for hybridization with a DIG-labeled anti-sense riboprobe for CaMKIIα mRNA. CaMKIIα mRNA was detected by mouse anti-DIG Fab fragment followed by Alexa568-labeled anti-mouse IgG (red), whereas GFP-NXF2 was detected using rabbit anti-GFP followed by Alexa488 labeled anti-rabbit IgG (green). Scale bars, 10 μm. Right: Fluorescent intensity profile along the X–Y axis indicated in the upper right image. () GFP-NXF2 and mRFP-Stau1 were co-expressed in cultured rat hippocampal neurons and their localizations were observed at 24 h after transfection by fluorescence confocal microscopy. The insert in the merged image is a blow-up of the region of the image indicated. Scale bar, 10 μm.