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NXF2 co-localizes with the components of RNA granules in dendrites of primary neurons

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posted on 31.12.2011, 07:18 by Keizo Takano, Takashi Miki, Jun Katahira, Yoshihiro Yoneda

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Taken from "NXF2 is involved in cytoplasmic mRNA dynamics through interactions with motor proteins"

Nucleic Acids Research 2007;35(8):2513-2521.

Published online 1 Apr 2007

PMCID:PMC1885657.

© 2007 The Author(s)

() GFP-NXF2-expressing neurons were processed for hybridization with Cy3-labeled oligo(dT) probe (red). The insert in the merged image is a blow-up of the region of the image indicated. Scale bars, 10 μm. () Left: GFP-NXF2-expressing neurons were processed for hybridization with a DIG-labeled anti-sense riboprobe for CaMKIIα mRNA. CaMKIIα mRNA was detected by mouse anti-DIG Fab fragment followed by Alexa568-labeled anti-mouse IgG (red), whereas GFP-NXF2 was detected using rabbit anti-GFP followed by Alexa488 labeled anti-rabbit IgG (green). Scale bars, 10 μm. Right: Fluorescent intensity profile along the X–Y axis indicated in the upper right image. () GFP-NXF2 and mRFP-Stau1 were co-expressed in cultured rat hippocampal neurons and their localizations were observed at 24 h after transfection by fluorescence confocal microscopy. The insert in the merged image is a blow-up of the region of the image indicated. Scale bar, 10 μm.

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